isolation of N. gonorrhoeae from sites of exposure (e.g., urethra, endocervix, throat, rectum) by culture (usually a selective medium) and demonstrating typical colonial morphology, positive oxidase reaction, and typical gram-negative morphology and.

What other specimen can be used for isolation of Neisseria gonorrhoeae?

Urethral specimens: Discharge from the meatus is preferred for the detection of N gonorrhoeae. If there is no meatal exudate in postpubertal patients, an intraurethral swab can be used for the detection of gonococci.

How do you test for Neisseria gonorrhoeae?

  1. Urine test. This can help identify bacteria in your urethra.
  2. Swab of affected area. A swab of your throat, urethra, vagina or rectum can collect bacteria that can be identified in a lab.

Is a selective medium for isolation of Neisseria gonorrhoeae?

GC-Lect, a new selective medium for the isolation of Neisseria gonorrhoeae which contains five antimicrobial agents, was evaluated with stock cultures and with 500 clinical specimens.

How do you grow N. gonorrhoeae?

A fastidious organism, N. gonorrhoeae requires enriched media in a CO2 atmosphere at 35 degrees to 37 degrees C for growth. In addition, N. gonorrhoeae expresses potent autolysins whose activity increases following glucose depletion during stationary phase, leading to cell death.

What specimens can be collected from patients with gonorrhea organisms?

Specimens collected for gonorrhea culture should be obtained by using swabs with plastic or wire shafts and rayon, Dacron, or calcium alginate tips. Other swab material such as wood shafts and cotton tips might be inhibitory or toxic to the organism and should be avoided.

What selective media is the most appropriate for N. gonorrhoeae?

For the isolation of gonococci, the selective culture medium containing colistin, vancomycin, nystatin and trimethoprim which is usually employed has been changed by substituting lincomycin for vancomycin. The best result was obtained if a concentration of 1/2 mug lincomycin/ml medium was used.

How should a specimen for Neisseria gonorrhoeae be transported to the laboratory?

gonorrhoeae is extremely susceptible to drying and temperature changes, direct plating of the specimen to gonococcal-selective media gives optimal results. Inoculated swabs should be placed in a transport system such as Amies medium with charcoal, transported to the laboratory immediately, and plated within 6 hours.

What body sample would you collect from a patient suspected with gonorrhea?

In a direct smear, a sample of body fluid is taken from the area where gonorrhea is suspected. In adults, this may include the urethra, the cervix, the rectum, or the eye.

Is Thayer-Martin Agar selective or differential?

Thayer-Martin agar is a selective medium used for the isolation of gonococci (Neisseria gonorrheae) from specimens containing a mixed flora of bacteria and/or fungi e.g. urogenital specimen. Modified Thayer-Martin (MTM) agar is a GC agar base containing vancomycin, colistin, nystatin, and trimethoprim lactate (VCNT).

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What media is isolate pathogenic Neisseria species?

Neisseria spp. are fastidious. Blood agar and chocolate medium (blood heated at 176–194°F/80–90°C) are suitable growth media. Bacterial colonies usually appear after 24–48 hours of growth.

Is Neisseria gonorrhoeae aerobic or anaerobic?

Abstract. Neisseria gonorrhoeae is generally considered to be an obligate aerobe; it can, however, grow in the absence of oxygen by anaerobic respiration by using nitrite as a terminal electron acceptor.

What does no Neisseria gonorrhoeae isolated mean?

In women, the infection can spread and cause pelvic inflammatory disease (PID). Normal Range : No Neisseria gonorrhoeae isolated. Interpretation : Normal. Neisseria gonorrhoeae culture results are negative (no growth) Abnormal.

WHO recommended treatment of uncomplicated Neisseria gonorrhoeae?

For treatment of uncomplicated urogenital, rectal, or pharyngeal gonorrhea, CDC recommends a single 500 mg IM dose of ceftriaxone (Box). For persons weighing ≥150 kg (300 lbs), a single 1 g IM dose of ceftriaxone should be administered.

What is the best test to differentiate Neisseria species?

Gonochek II is a tube test that is designed to differentiate between Neisseria lactamica, N. meningitidis, N. gonorrhoeae and Moraxella catarrhalis. The enzymes produced by these species are detected in a single test by the production of colored endproducts from colorless substrates.

Does Neisseria gonorrhoeae grow on blood agar?

Gonococci are not able to grow on common blood agar. … agar is used for growing fastidious respiratory bacteria, such as Haemophilus influenzae and Neisseria meningitidis.

How do you grow Neisseria meningitidis?

meningitidis are gram-negative, coffee-bean shaped diplococci that may occur intracellularly or extracellularly in PMN leukocytes. N. meningitidis is a fastidious organism, which grows best at 35-37°C with ~5% CO2 (or in a candle-jar). It can grow on both a blood agar plate (BAP) and a chocolate agar plate (CAP).

Is Neisseria gonorrhoeae the same as gonorrhea?

Neisseria gonorrhoeae, a Gram-negative coccus, causes gonorrhea, a sexually transmitted infection typified by genital or anorectal infection and discharges. One case report described transmission of gonococcal pharyngitis by intimate kissing.

Does Neisseria gonorrhoeae grow on sheep blood agar?

gonorrhoeae; however, fair growth of the bacteria on blood agar as well as on chocolate agar made that identification more suspicious because it is known that some N. gonorrhoeae strains can grow, but more slowly, and less well, on commercially available sheep blood agar [13].

What grows MacConkey Agar?

Altogether, MacConkey agar only grows gram-negative bacteria, and those bacteria will appear differently based on their lactose fermenting ability as well as the rate of fermentation and the presence of a capsule or not.

Is Neisseria gonorrhoeae motile or nonmotile?

Neisseria gonorrhoeae is an aerobic gram-negative, non-spore forming, non-motile, encapsulated, and non-acid-fast bacteria, which appear in kidney bean shape under the microscope.

What type of organism is Neisseria gonorrhoeae?

Neisseria gonorrhoeae is a Gram-negative, obligate, fastidious, diplococcus bacteria. Like all Gram-negative bacteria, N. gonorrhoeae possesses a cell envelope composed of an inner cytoplasmic membrane, a middle layer of peptidoglycan, and an outer membrane [92].

What physical examination and laboratory procedure are required to establish a diagnosis of gonorrhea?

Laboratory Testing A nucleic acid amplification test (NAAT) must be performed for screening and panel physicians must use the least-invasive specimen type. For asymptomatic applicants without risk factors, a urine specimen (men or women) or a self-collected vaginal swab (women) must be used for the NAAT.

Does a urine test show gonorrhea?

Testing for gonorrhea looks for the presence of Neisseria gonorrhoeae (gonorrhea bacteria) in your body. Urine samples can be used to test both men and women. The most accurate way to perform the test is to collect first-catch urine.

Can routine urine test detect gonorrhea?

Currently, only gonorrhea and chlamydia are commonly tested for by using urine samples. However, there are other STIs that can be tested for this way.

How long after treatment does gonorrhea test positive?

Type of STIPathogen typeWhen to retest after treatmentGonorrheabacteriaTest 2 weeks after treatment, or 2 weeks later after exposure if the first test is negativeHerpesvirusNoneHPVvirusNoneHepatitisvirusRetest 6 months later

What media should be used to isolate Neisseria meningitidis?

Blood culture brothBlood culture medium is used to grow N. meningitidis, S. pneumoniae, and H. influenzae.

What is the aim of the gonococcal Isolate Surveillance Project GISP )?

The Gonococcal Isolate Surveillance Project (GISP) was established in 1986 to monitor antimicrobial susceptibility trends in N. gonorrhoeae strains in the United States and to establish a rational basis for the selection of gonococcal therapies.

Which method has the best sensitivity and specificity for gonorrhea?

As with chlamydia, NAATs are now considered the gold standard for diagnosing gonorrhea. The USPSTF found that testing for gonorrhea using NAATs had a sensitivity ranging from 90 to 100 percent and a specificity greater than 97 percent.

How do you differentiate Neisseria gonorrhoeae and Neisseria meningitidis?

N. gonorrhoea is kidney shaped with apposing ends concave. N. meningitidis is semicircular diplococcus with flat apposing ends.

Does Neisseria sicca grow on Thayer Martin?

Unlike N. meningitidis and N. gonorrhoeae, the other Neisseria species (except N. lactamica) generally do not grow on selective media such as Thayer–Martin agar.